Isolation:Article Title: Targeted delivery of antisense oligonucleotides to pancreatic β-cells.
Article Snippet: Islets were dispersed using TrypLE Express (Thermo Fisher Scientific, #12605036) and plated in 96-well PDL-coated plates. .. Human primary islets were purchased from Prodo Laboratories Inc., providing islets isolated from donor pancreas obtained from deceased individuals with research consent from organ procurement organizations. .. The use and storage of human islets were performed in compliance with the Declaration of Helsinki, International Conference on Harmonisation/Good Clinical Practice, and AstraZeneca code D ow nloaded from https://w w w .science.org on January 23, 2024 Ämmälä et al., Sci.
Recombinant:Article Title: Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing
Article Snippet: .. N/A Human primary islets Prodo Laboratories, Inc 11 Chemicals, Peptides, and Recombinant Proteins 2-mercaptoethanol Sigma Aldrich M3148 2-Propanol 99,7% Panreac Quimica 11610901214 30%(w/v) Acrylamide: 0.8% /9w/v) Bis-Acrylamide Stock Solution (37.5:1) ProtoGel EC-890 Acetic Acid Fisher Scientific 100063.1000 Agarose Electrophoresis Grade Apollo Scientific BIA1176 Aprotinin Sigma Aldrich A1153 (Continued on next page) Cell Reports Medicine 1, 100125, October 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aqua Live Dead Life Technologies L34957 Blotting-Grade Blocker BioRad 170-6404 BD Cytofix BD BioSciences 51-2090KZ BD Perm/Wash BD BioSciences 51-2091KZ Bovine Serum Albumin Sigma Aldrich A7906 Bradford Protein Assay BioRad 5000001 Bromophenol Blue Sigma Aldrich B0126 Citric Acid 500 g Sigma Aldrich C0759 Chloroform Sigma Aldrich C2432 Collagenase P Roche 11213857001 Coomassie Brilliant Blue BioRad 161-0436 CompleteTM, EDTA-free Protease Inhibitor Cocktail Roche COEDTAF-RO D-Glucose 250 g Sigma Aldrich G7528 DAPI 4.6-diamidino-2-phenylindole 5 mg Sigma Aldrich D9542 DMEM Life Technologies, S.A. 31885-049 DMSO Sigma Aldrich D8418 DNase I recombinant, RNase-free Roche 04716728001 Doxycycline 1g Sigma Aldrich D9891 DTT (Dithiothreitol) Sigma Aldrich D9779 EcoRI Digestion Enzyme New England Biolabs R0101S EcoRV Digestion Enzyme New England Biolabs R3195M EDTA (Ethylenediaminetetraacetic acid) Sigma Aldrich ED EGTA (Ethylene glycol-bis(2aminoethylether)-N, N, N, N-tetraacetic acid Sigma Aldrich E3889 Ethanol 96% Panreac Quimica 141085.1214 Fetal Bovine Serum Sigma Aldrich F7524-500ML FITC/Dextran Sigma Aldrich 46945 Formalin solution 10% Panreac Quimica S.L.U.
Electrophoresis:Article Title: Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing
Article Snippet: .. N/A Human primary islets Prodo Laboratories, Inc 11 Chemicals, Peptides, and Recombinant Proteins 2-mercaptoethanol Sigma Aldrich M3148 2-Propanol 99,7% Panreac Quimica 11610901214 30%(w/v) Acrylamide: 0.8% /9w/v) Bis-Acrylamide Stock Solution (37.5:1) ProtoGel EC-890 Acetic Acid Fisher Scientific 100063.1000 Agarose Electrophoresis Grade Apollo Scientific BIA1176 Aprotinin Sigma Aldrich A1153 (Continued on next page) Cell Reports Medicine 1, 100125, October 20, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Aqua Live Dead Life Technologies L34957 Blotting-Grade Blocker BioRad 170-6404 BD Cytofix BD BioSciences 51-2090KZ BD Perm/Wash BD BioSciences 51-2091KZ Bovine Serum Albumin Sigma Aldrich A7906 Bradford Protein Assay BioRad 5000001 Bromophenol Blue Sigma Aldrich B0126 Citric Acid 500 g Sigma Aldrich C0759 Chloroform Sigma Aldrich C2432 Collagenase P Roche 11213857001 Coomassie Brilliant Blue BioRad 161-0436 CompleteTM, EDTA-free Protease Inhibitor Cocktail Roche COEDTAF-RO D-Glucose 250 g Sigma Aldrich G7528 DAPI 4.6-diamidino-2-phenylindole 5 mg Sigma Aldrich D9542 DMEM Life Technologies, S.A. 31885-049 DMSO Sigma Aldrich D8418 DNase I recombinant, RNase-free Roche 04716728001 Doxycycline 1g Sigma Aldrich D9891 DTT (Dithiothreitol) Sigma Aldrich D9779 EcoRI Digestion Enzyme New England Biolabs R0101S EcoRV Digestion Enzyme New England Biolabs R3195M EDTA (Ethylenediaminetetraacetic acid) Sigma Aldrich ED EGTA (Ethylene glycol-bis(2aminoethylether)-N, N, N, N-tetraacetic acid Sigma Aldrich E3889 Ethanol 96% Panreac Quimica 141085.1214 Fetal Bovine Serum Sigma Aldrich F7524-500ML FITC/Dextran Sigma Aldrich 46945 Formalin solution 10% Panreac Quimica S.L.U.
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